Schuster Colloquium 1st October - Professor Paola Borri, University of Cardiff
All staff and students are invited to attend the Schuster Colloquium on Wednesday 1st October 2014 at 14.30 in the Rutherford Lecture Theatre, Schuster Building, when Professor Paola Borri, University of Cardiff, will be speaking on the following topic: "Shedding new light on cells with coherent multiphoton microscopy"
Optical microscopy is an indispensable tool in cell biology, and is still the only practical means of obtaining high spatial and temporal resolution within living cells and tissues. Coherent Antistokes Raman Scattering (CARS) microscopy has recently emerged as a new chemically-specific label-free multiphoton microscopy technique. We have developed in our laboratory two home-built CARS microscopes. One of these features a single 5 femtosecond Ti:Sa source, capable of exciting a wide vibrational range, thus enabling powerful hyperspectral microscopy analysis. Furthermore, we have invented and demonstrated a novel imaging modality, based on the resonant Four-Wave Mixing (FWM) of colloidal nanoparticles. Results of this work showed that gold nanoparticles can be used as alternative labels beyond traditional fluorescence by exploiting their resonant FWM, to achieve a novel coherent multiphoton microscopy modality free from background, and with a spatial resolution significantly surpassing the one-photon diffraction limit. I will present our latest progress with both techniques and their applications to cell imaging.